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71 Cards in this Set

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Normal flora or intestines; lactose fermenter; gram - rod.
E. coli
Acid fast; saprophyte; gram+ rod
Mycobacterium smegmatis
keyword= acid fast. What are acid-fast.
VP; positive; citrate positive; gram - rod.
Enterobacter aerogenes.
Hint= ONLY VP may Enter but the orange citrus may give you diarrhea.
Grows on mannitol "salt" agar; facultative halophile; lipase positive; most antibiotic sensitive organism used in lab; gram+ cocci.
Staphylococcus aureus.
Aerobic sporeformer; caseinase positive; gram+ rod.
Bacillus subtilis.
hint= Casey is positively full of BS. Yeah, a real fun guy.
Urease positive; hydrogen sulfide positive; gram- rod.
Proteus vulgaris
hint= Urease and H. sulfide are vulgar.
"Obligate halophile"; requires 15% salt.
Halobacterium salinarium.
Opportunistic bacteria; blue-green pigment aerobic; non-fermentative; lipase positive; oxidase positive; gram- rod.
Pseudomonas aeruginosa.
hint= blue-green is psychodelic.
Produces a "red pigment" at room temperature; gram- rod.
Serratia marcesans.
hint= like a Serrated sunset.
Citrate positive; produces alkaline results in fermentation media; gram- rod.
Salmonella
hint= Salmon drink milk.Orange-Fish (citrate) swim in milk/alkaline.
Anaerobic sporeformer; gram+ rod.
Clostridium sporogenes.
hint= girls clostridium do anaerobics.
Produces a yellow pigment.
Micrococcus lutea.
hint= small yellow tea
Phenol is used as a ___.
control
Yellow-acid, gas; maybe a bubble; phenol red/ Durham tube.
Fermentation
Keyword= bubble therefore there's carbondioxide. Why the production of carbon dioxide?
Red; MRVP broth; "formic" hydrogen; methyl red indicator
Mixed acid fermentation.
Hint= Mr. VP ferments mixed acid and butanediol.
Red color developes;
"Barritt's" reagent A and B;
Vogues-Proskauer test
hint= mrVP
Bubbles; hydrogen peroxide; nutient agar.
Catalase production.
Keyword= catalase
Colonies turn purple; Oxidase test reagent; oxidase; trypticase soy agar (TSA)
Oxidase Production
Hint= what is it testing for? It's an Oxidase test reagent.
Red color developes; nitrite reagents A and B;
Nitrate reduction
Clear areas next to growth; iodine; amylase.
Starch Hydrolysis.
Clear areas next to growth; no test reagents; skim milk agar.
Casein Hydrolysis
Keyword=Casey may be teased by Jello but Casey loves milk.
"Dark blue" precipitate; no test reagent; "lipase"; spirit blue agar.
Fat Hydrolysis.
keyword=BLUE FAT melt.
Fuschia color; no test reagent; urea broth.
Urea hydrolysis.
Keyword = urease
Red if positive for indole; Kovac's reagent; What is produced or occurs or what gets hydrolyzed?
Tryptophan hydrolysis.
keyword= Bob Dole is trippin on Cojac.
Agar turns black; no test reagent; triple sugar iron agar.
Hydrogen sulfide production
hint= Black from the pits of hell; fire and brimstone.
Prussian blue color change; no reagents; ___ medium.
Citrate production
keyword= Prussians like blue but want a color change to citrus orange.
Deep green color develops; 10% ferric chloride; ___ agar.
Phenylalanine deamination
Keyword= Phen.
hint= color changes from color to green tint.
growth near top of broth-aerobe; thioglycollate broth.
Oxygen requirements
hint= there are aerobes therefore require ___.
2, 3-butanediol "Fermentation"
MR-VP broth
hint= Mr. VP ferments butane like wine.
carbohydrate fermentation
Kliger's iron agar
hint= ironing sugar.
Casein hydrolysis - What's the medium?
Medium : Skim milk agar
hint= Casey's medium length milky white skin.
Citrate utilization - What's the medium?
Medium : Simmon's citrate agar
hint= Simon says utilize citrate.
What's the medium used with Hydrogen Sulfide production?
Medium : Sims medium and Kliger's iron
hint= Blackened iron and sims medium.
Mixed acid fermentation - What's the medium?
Medium : MR-VP broth
hint= Mr. VP ferments mixed acid and butanediol
Triglyceride hydrolysis - What's the "medium"?
Medium : Spirit blue agar
hint= Triglyceride's medium is ___ ___ ___ .
What is the "medium" used for Tryptophan degradation?
Medium : Tryptone broth
keyword= The tone is medium.
Tip-toe through the medium is so degrading.
What's the "reagent" for 2, 3 butaNediol fermentation?
Alpha naphthol
hint= The FIRE of Alpha.
NOT the medium but it is asking for the reagent.
What's the "reagent" for the catalase "test"?
Hydrogen peroxide
hint= reagent to test for catalase.
What's the "reagent" for "mixed" acid fermentation?
Methyl red.
hint= mixed folk do Meth
Like Agent Orange it's
Methyl Red.
What's the "reagent" for nitrate reduction?
Sulfanilic acid.
hint= Licking nitrate.
What's the "reagent" for oxidase test?
NNNN, - tetramethyl-phenylenediamine dihydrochloride.
hint= reagents: like O2 there's N4.
What's the "reagent" for pheylalanine deamination?
Ferric chloride.
hint= Ferrits are deaminated.
What's the "reagent" for starch hydrolysis?
Gram's iodine.
hint= That agent is a white guy.
What's the "reagent" for tryptophan degradation?
Kovac's reagent
Cojak is so degrading.. what a trip.
What's the "enzyme" for this reaction: Casein hydrolysis.
Protease.
hint= Teasing Casey with Jello.
What's the enzyme for this reaction: Gelatin liquifaction.
Protease
hint= Teasing me with jello pudding.
What's the "enzyme" for this reaction: hydrogen sulfide production.
Cysteine desulfurase.
hint= H.S. produced the cystein chapel.
What's the ENZYME for this reaction: indole.
Tryptophanase
hint= The Bob Dole trip.
What's the ENZYME for this reaction: starch hydrolysis.
Amylase.
hint= Amy loves spagetti.
What's the ENZYME for this reaction: Triglyceride hydrolysis.
Lipase.
hint= Fat lipped enzyme
What's the enzyme for this reaction: Urea hydrolysis.
Urease.
hint = erasing urea.
What's the product for this reaction: Catalase.
Molecular oxygen.
Hint= Catalase hydrolysis hydrogen peroxide thus creating the product "oxygen".
What's the product for this reaction: Phenylalanine deamination.
Phenylpyruvic acid.
What's the product for this reaction: Triglyceride hydrolysis.
Fatty acids
keyword= triglyceride hydrolysis is melted fat acid.
What's the product for the reaction Urea hydrolysis.
Ammonia
Hint = ureA.
What's the product for this reaction: Voges-Proskauer test.
2, 3-Butanediol.
Hint = Caveman VP produces fire.
What length of time is required for the destruction of S. aureua? B. megaterium? Why does it take longer for B. megaterium?
S. aureus: 10 minutes
S. aureus: 10 minutes
S. aureus: 10 minutes
S. aureus: 10 minutes
B. megaterium: 60 min.
because of endospores.
Why leave the cover on one set of petri dishes when exposing to US light?
To use as a control.
Describe what happens when DNA is exposed to UV light?
Thymines cross-link to form thymine dymers.
How do cells repair UV damage?
SOS: enymatically rfemoves the dimers and inserts pyrimidine molecules in their place. It cannot repair all damage. It makes errors by inserting bases.
Differentiate between antimicrobials and antibiotics.
Antimicrobials
kill/control bacterial "GROWTH".
Antibiotics
made from other organisms damage the CELL WALL of bacteria.
A narrow spectrum antimicrobe ___ ___.
controls few.
A broad spectrum antimicrobic.
Controls many.
Which antiseptics were most effective at controlling the growth of S. aureus and P. aeruginosa?
Formaldehyde for both.
What factors influence the zone of inhibition?
amount of exposure and sample.
Differentiate between antiseptics and disinfectants.
Antiseptics
are gentle enough to use on living tissue but don't destroy endospores.
Disinfectants
are used on inanimate objects and damage tissues.
You disinfect inanimate objects
NOT PEOPLE.
Differentiate between bacteriostatic and bacteriocidal.
Bacteriostatic are agents inhibit bacterial growth.
bacteriocidal are agents that kill bacteria.
An excellent "broad"-spectrum antimicrobic.
Gentamycin.
hint= Gentlemen look at broads.
Loves sugar.
Osmophile.
This microorganism tolerates a broad range of salt concentrations.
Staph-ylo-coccus
What is the optimum temperature for E.coli?
38-42 degrees C.