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9 Cards in this Set

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The foundational strategy that is the basis of the Sanger sequencing technique is


A. Using ATP production resulting from nucleotide incorporation to drive a luciferase reaction that produces detectable light


B. Using dideoxyribonucleotide bases that halt DNA synthesis after incorporation into the growing DNA strand


C. Affixing short pieces of DNA fragments to beads which are then individually amplified through PCR


D. Creating small segments of DNA that are sequenced and then assembled using powerful computer algorithms

B. Using dideoxy-ribonucleotide bases that halt DNA synthesis after incorporation into the growing DNA strand

The Sanger method of sequencing uses ALL of the following EXCEPT:


A. Dideoxynucleotides


B. DNA polymerase


C. A short oligo primer


D. Aeoxynucleotides


E. Restriction enzymes

E. Restriction enzymes

Microarray analysis may be used to study:


A. Gene expression in cells grown under different conditions


B. Protein expression in cells grown under different conditions


C. Rate of chromosomal replication in cells


D. Enzyme activity in cells


E. Growth rate of cells when grown under different conditions

A. Gene expression in cells grown under different conditions

(True/False) A transcriptome refers to all the proteins produced in a single cell.

False

Match the technique with its target molecule:


A. Microarray


B. 2D-PAGE


C. Northern blot


D. 454 sequencing

A: mRNA


B: Proteins


C: mRNA


D: DNA

Which of these can be used to determine protein structure?


A. Amino acid sequencing


B. X-ray crystallography and NMR


C. Polyacrylamide gel electrophoresis (PAGE)


D. Denaturing gel electrophoresis


E. Mass spectrometry

B. X-ray crystallography and NMR

Which of these is TRUE regarding horizontal gene transfer?


A. It does not result in permanent change


B. It does not play a role in microbial evolution


C. It does not result in any significant consequences for the organism involved


D. It is always occurring in nature


E. It does not alter the genome of the organism

D. It is always occurring in nature

(True/False) Pyrosequencing takes its name from the fact that this technique detects light that is emitted each time a new nucleotide base is added to the growing DNA strand.

True

What is an open-reading frame?


A. A non-translated region on the chromosome


B. An unknown gene


C. A gene that no longer has a function


D. The protein encoding sequence of a gene


E. The intron region of a gene

D. The protein encoding sequence of a gene